Anticorps Monoclonal anti-SETD1A

• Phare
• Validé par KD/KO

SETD1A Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C2G12

N° de cat : 67936-1-PBS

Synonymes

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

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Galerie de données de validation

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  WB analysis using 67936-1-Ig (same clone as 67936-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67936-1-Ig (same clone as 67936-1-PBS)

  WB result of SETD1A antibody (67936-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETD1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67936-1-Ig (same clone as 67936-1-PBS)

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at a dilution of 1:3000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67936-1-Ig (same clone as 67936-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67936-1-Ig (same clone as 67936-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67936-1-Ig (same clone as 67936-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67936-1-Ig (same clone as 67936-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67936-1-Ig (SETD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67936-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67936-1-PBS cible SETD1A dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SETD1A Protéine recombinante Ag30424
• Nom complet: SET domain containing 1A
• Masse moléculaire calculée: 186 kDa
• Poids moléculaire observé: 270 kDa
• Numéro d’acquisition GenBank: NM_014712
• Symbole du gène: SETD1A
• Identification du gène (NCBI): 9739
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).

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