Anticorps Polyclonal de lapin anti-SETD3

SETD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28692-1-PBS

Synonymes

C14orf154, SET domain containing 3

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Galerie de données de validation

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  WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28692-1-PBS cible SETD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SETD3 Protéine recombinante Ag30017
• Nom complet: SET domain containing 3
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: NM_032233
• Symbole du gène: SETD3
• Identification du gène (NCBI): 84193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).

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