Anticorps Polyclonal de lapin anti-SETD7

• Phare
• Validé par KD/KO

SETD7 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24840-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 24840-1-AP (same clone as 24840-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 24840-1-AP (same clone as 24840-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 24840-1-AP (same clone as 24840-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 24840-1-AP (same clone as 24840-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24840-1-AP (same clone as 24840-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24840-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24840-1-PBS cible SETD7 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SETD7 Protéine recombinante Ag18219
• Nom complet: SET domain containing (lysine methyltransferase) 7
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC121055
• Symbole du gène: SETD7
• Identification du gène (NCBI): 80854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

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