Anticorps Polyclonal de lapin anti-SF1

SF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11635-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11635-1-AP (same clone as 11635-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11635-1-AP (SF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 11635-1-AP (same clone as 11635-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11635-1-AP (SF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11635-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11635-1-PBS cible SF1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF1 Protéine recombinante Ag2209
• Nom complet: splicing factor 1
• Masse moléculaire calculée: 638 aa, 68 kDa
• Poids moléculaire observé: 65-71 kDa
• Numéro d’acquisition GenBank: BC020217
• Symbole du gène: SF1
• Identification du gène (NCBI): 7536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SF1 is a nuclear pre-mRNA splicing factor, it specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The molecular weight of SF1 ranges from 59 kDa to 71 kDa due to alternative splicing.

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