Anticorps Recombinant de lapin anti-SF3A1

SF3A1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250768F4

N° de cat : 86170-2-PBS

Synonymes

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

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Galerie de données de validation

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  WB analysis using 86170-2-RR (same clone as 86170-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 86170-2-RR (same clone as 86170-2-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86170-2-RR (same clone as 86170-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SF3A1 antibody (86170-2-RR, Clone: 250768F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86170-2-PBS in a different storage buffer formulation.

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Informations sur le produit

86170-2-PBS cible SF3A1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SF3A1 Protéine recombinante Ag8196
• Nom complet: splicing factor 3a, subunit 1, 120kDa
• Masse moléculaire calculée: 793 aa, 89 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC007684
• Symbole du gène: SF3A1
• Identification du gène (NCBI): 10291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

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