Anticorps Recombinant de lapin anti-SF3B1

SF3B1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243145C6

N° de cat : 85640-4-RR

Synonymes

PRP10, SAP 155, SAP155, SF3b155

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Galerie de données de validation

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  WB analysis using 85640-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85640-4-RR (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85640-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85640-4-RR against Human SF3B1 were performed. The affinity constant is 6.07 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules HeLa, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85640-4-RR cible SF3B1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SF3B1 Protéine recombinante Ag26689
• Nom complet: splicing factor 3b, subunit 1, 155kDa
• Masse moléculaire calculée: 146 kDa
• Poids moléculaire observé: 146 kDa
• Numéro d’acquisition GenBank: NM_012433
• Symbole du gène: SF3B1
• Identification du gène (NCBI): 23451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

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