Anticorps Monoclonal anti-SF3B3

SF3B3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H7F5

N° de cat : 67469-1-PBS

Synonymes

SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit, KIAA0017

---

Galerie de données de validation

• 

  WB analysis using 67469-1-Ig (same clone as 67469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67469-1-Ig (SF3B3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67469-1-Ig (SF3B3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 67469-1-Ig (same clone as 67469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (67469-1-Ig, Clone: 2H7F5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67469-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67469-1-PBS cible SF3B3 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SF3B3 Protéine recombinante Ag6315
• Nom complet: splicing factor 3b, subunit 3, 130kDa
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 130-135 kDa
• Numéro d’acquisition GenBank: BC003146
• Symbole du gène: SF3B3
• Identification du gène (NCBI): 23450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

{{ptg:RelatedPrimaryAntibodies}}