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Anticorps Recombinant de lapin anti-SF3B4

SF3B4 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243098D11

N° de cat : 85663-5-PBS

Synonymes

Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49, SF3b49, SF3b50

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using SF3B4 Recombinant antibody (85663-5-RR)

    WB analysis using 85663-5-RR (same clone as 85663-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85663-5-RR (SF3B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85663-5-PBS in a different storage buffer formulation.

Informations sur le produit

85663-5-PBS cible SF3B4 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène SF3B4 Protéine recombinante Ag0737
Nom complet splicing factor 3b, subunit 4, 49kDa
Masse moléculaire calculée 44 aa, 3 kDa
Poids moléculaire observé49 kDa
Numéro d’acquisition GenBankBC004273
Symbole du gène SF3B4
Identification du gène (NCBI) 10262
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)

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