Anticorps Recombinant de lapin anti-SF3B4

SF3B4 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243098D11

N° de cat : 85663-5-RR

Synonymes

Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49, SF3b49, SF3b50

---

Galerie de données de validation

• 

  WB analysis using 85663-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85663-5-RR (SF3B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293T, cellules Jurkat, cellules NIH/3T3, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85663-5-RR cible SF3B4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SF3B4 Protéine recombinante Ag0737
• Nom complet: splicing factor 3b, subunit 4, 49kDa
• Masse moléculaire calculée: 44 aa, 3 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC004273
• Symbole du gène: SF3B4
• Identification du gène (NCBI): 10262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)

{{ptg:RelatedPrimaryAntibodies}}