Anticorps Monoclonal anti-SF4

SF4 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G9D8

N° de cat : 68319-1-PBS

Synonymes

SURP and G-patch domain-containing protein 1, SUGP1, Splicing factor 4, RNA-binding protein RBP, F23858

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Galerie de données de validation

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  WB analysis using 68319-1-Ig (same clone as 68319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 68319-1-Ig (same clone as 68319-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 68319-1-Ig (SF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 68319-1-Ig (same clone as 68319-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug SF4 Monoclonal antibody (68319-1-Ig, Clone:1G9D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68319-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68319-1-PBS cible SF4 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SF4 Protéine recombinante Ag8014
• Nom complet: splicing factor 4
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 55 kDa, 72 kDa
• Numéro d’acquisition GenBank: BC002986
• Symbole du gène: SF4
• Identification du gène (NCBI): 57794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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