Anticorps Polyclonal de lapin anti-SFRP1

• Phare
• Validé par KD/KO

SFRP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 26460-1-AP

Synonymes

FRP, FRP 1, FRP1, FrzA, SARP 2, SARP2, sFRP 1, SFRP1

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Galerie de données de validation

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  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26460-1-AP (SFRP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26460-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26460-1-AP (SFRP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26460-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26460-1-AP (SFRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules NIH/3T3, cellules SH-SY5Y, cellules U2OS, tissu rénal de rat, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

26460-1-AP cible SFRP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRP1 Protéine recombinante Ag24844
• Nom complet: secreted frizzled-related protein 1
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC036503
• Symbole du gène: SFRP1
• Identification du gène (NCBI): 6422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Genes Dis; Naked cuticle homolog 2 controls the differentiation of osteoblasts and osteoclasts and ameliorates bone loss in ovariectomized mice; Authors - Liying Shan
human	WB	APL Bioeng; Oscillatory shear stress-induced downregulation of TET1s injures vascular endothelial planar cell polarity by suppression of actin polymerization; Authors - Kai Qu ; KD Validated
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
human	IHC	Transl Oncol; Excavating regulated cell death signatures to predict prognosis, tumor microenvironment and therapeutic response in HR+/HER2- breast cancer; Authors - Shuangshuang Mao
rat	IHC	Cells; Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells.; Authors - Keita Ipposhi
		Cell Rep; Demyelination Regulates the Circadian Transcription Factor BMAL1 to Signal Adult Neural Stem Cells to Initiate Oligodendrogenesis.; Authors - Suihong Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-14-2023)

There are doubts about if this antibody is working in WB. There are some non-specific bands and the supposed band is very weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2, mouse liver, mouse kidney, mouse brain

SFRP1 Antibody Western Blot validation (1:2000 dilution) in HepG2, mouse liver, mouse kidney, mouse brain (Cat no:26460-1-AP)

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