Anticorps Monoclonal anti-SFRP2

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F3A1

N° de cat : 66328-1-PBS

Synonymes

1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1

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Galerie de données de validation

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  WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66328-1-Ig (same clone as 66328-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66328-1-Ig (same clone as 66328-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51471-1

  Cytometric bead array sample test of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS.

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  Cytometric bead array standard curve of MP51471-1

  Cytometric bead array standard curve of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS. Standard:Ag18840. Range: 0.781-100 ng/mL.

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Informations sur le produit

66328-1-PBS cible SFRP2 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SFRP2 Protéine recombinante Ag18840
• Nom complet: secreted frizzled-related protein 2
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC008666
• Symbole du gène: SFRP2
• Identification du gène (NCBI): 6423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

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