Anticorps Polyclonal de lapin anti-SFRS7

• Phare
• Validé par KD/KO

SFRS7 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11044-1-AP

Synonymes

9G8, AAG3, SFRS7, Splicing factor 9G8, SRSF7

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Galerie de données de validation

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  WB analysis using 11044-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11044-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 11044-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11044-1-AP

  Immunofluorescent analysis of HepG2 cells, using SFRS7 antibody 11044-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules SKOV-3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11044-1-AP cible SFRS7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRS7 Protéine recombinante Ag1526
• Nom complet: splicing factor, arginine/serine-rich 7, 35kDa
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC000997
• Symbole du gène: SFRS7
• Identification du gène (NCBI): 6432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Cell; C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.; Authors - Kyung-Ha Lee
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan ; KD Validated
human	WB	Nucleic Acids Res; Exonic splicing code and protein binding sites for calcium.; Authors - Reuben J Pengelly ; KD Validated
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun ; KD Validated
human	WB,IF	Genes Genomics; SRSF7 is a promising prognostic biomarker in hepatocellular carcinoma and is associated with immune infiltration; Authors - Wei Shen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human cancer cell line