Anticorps Polyclonal de lapin anti-SFRS9

• Phare
• Validé par KD/KO

SFRS9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 17926-1-AP

Synonymes

SRSF9, SRP30C, Splicing factor, arginine/serine-rich 9, Serine/arginine-rich splicing factor 9, Pre-mRNA-splicing factor SRp30C

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Galerie de données de validation

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  WB analysis of HEK-293 using 17926-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17926-1-AP (SFRS9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 17926-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17926-1-AP (SFRS9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du col de l'utérus humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17926-1-AP cible SFRS9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRS9 Protéine recombinante Ag12347
• Nom complet: splicing factor, arginine/serine-rich 9
• Masse moléculaire calculée: 221 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC093973
• Symbole du gène: SFRS9
• Identification du gène (NCBI): 8683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFRS9 is a member of the serine/arginine (SR) splicing factor family, which contains an N-terminal RNA recognition motif (RRM), a glycine-rich region, an internal region homologous to the RRM, and a long (315-amino-acid) C-terminal domain composed predominantly of alternating serine and arginine residues. SRSF9 has been linked to the occurrence and progression of hepatocellular carcinoma (HCC), colorectal cancer (CRC) and oral squamous cell carcinoma (OSCC). In HCC and CRC, the knockdown of SRSF9 inhibited the proliferation and migration of cancer cells, cell cycle progression and colony formation of cancer cells (PMID: 34336668,35971121).

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
human	WB	J Cell Biol; Stress granules regulate stress-induced paraspeckle assembly.; Authors - Haiyan An
human	WB	Sci Signal; ADAR2-mediated Q/R editing of GluA2 in homeostatic synaptic plasticity; Authors - Lucy Peterson ; KD Validated
human	WB	Hum Mutat; Antisense oligonucleotides modulate dopa decarboxylase function in aromatic l-amino acid decarboxylase deficiency.; Authors - Chi-Ren Tsai
human	WB	J Cancer Res Clin Oncol; SRSF9 promotes cell proliferation and migration of glioblastoma through enhancing CDK1 expression; Authors - Chunyuan Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human cancer cell line

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