Anticorps Polyclonal de lapin anti-SFXN1

• Phare
• Validé par KD/KO

SFXN1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12296-1-AP

Synonymes

SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

---

Galerie de données de validation

• 

  WB analysis using 12296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12296-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12296-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12296-1-AP (SFXN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12296-1-AP

  IP Result of anti-SFXN1 (IP:12296-1-AP, 4ug; Detection:12296-1-AP 1:800) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12296-1-AP (SFXN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 12296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12296-1-AP (SFXN1 Antibody) at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12296-1-AP cible SFXN1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFXN1 Protéine recombinante Ag2985
• Nom complet: sideroflexin 1
• Masse moléculaire calculée: 35.6 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC020517
• Symbole du gène: SFXN1
• Identification du gène (NCBI): 94081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula ; KO Validated
mouse	IHC,IF,IP	J Proteome Res; The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.; Authors - Fowler Stephanie L SL
human	WB	Cell Rep; The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.; Authors - Michelle Grace Acoba ; KO Validated
mouse	WB	Front Immunol; YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis; Authors - Jing Zhang
human	IHC,WB	Sci Rep; Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma; Authors - Kohei Yagi
mouse,rat	IHC,WB	Sci Total Environ; Ferritinophagy activation and sideroflexin1-dependent mitochondrial iron overload contribute to patulin-induced cardiac inflammation and fibrosis; Authors - Wen-Chang Sun ; KD Validated