Anticorps Monoclonal anti-SFXN1

• Phare
• Validé par KD/KO

SFXN1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D9F1

N° de cat : 68024-1-PBS

Synonymes

2D9F1, sideroflexin 1, Sideroflexin-1, TCC, Tricarboxylate carrier protein

---

Galerie de données de validation

• 

  WB analysis using 68024-1-Ig (same clone as 68024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68024-1-Ig (SFXN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 68024-1-Ig (same clone as 68024-1-PBS)

  WB result of SFXN1 antibody (68024-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN1 transfected MCF-7 cells. This data was developed using the same antibody clone with 68024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 68024-1-Ig (same clone as 68024-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SFXN1 antibody (68024-1-Ig, Clone: 2D9F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68024-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50630-2

  Cytometric bead array standard curve of MP50630-2, SFXN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68024-1-PBS. Detection antibody: 68024-4-PBS. Standard:Ag2985. Range: 0.781-100 ng/mL.

---

Informations sur le produit

68024-1-PBS cible SFXN1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SFXN1 Protéine recombinante Ag2985
• Nom complet: sideroflexin 1
• Masse moléculaire calculée: 35.6 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC020517
• Symbole du gène: SFXN1
• Identification du gène (NCBI): 94081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

{{ptg:RelatedPrimaryAntibodies}}