Anticorps Polyclonal de lapin anti-SGK1

• Phare
• Validé par KD/KO

SGK1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 23394-1-AP

Synonymes

EC:2.7.11.1, Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK

---

Galerie de données de validation

• 

  IP experiment of HEK-293 using 23394-1-AP

  IP result of anti-SGK1 (IP:23394-1-AP, 4ug; Detection:23394-1-AP 1:600) with HEK-293 cells lysate 1480ug.

• 

  IHC staining of human pancreas using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 23394-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23394-1-AP (SGK1 Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 23394-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGK1 antibody (23394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

23394-1-AP cible SGK1 dans les applications de IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGK1 Protéine recombinante Ag17888
• Nom complet: serum/glucocorticoid regulated kinase 1
• Masse moléculaire calculée: 431 aa, 49 kDa
• Numéro d’acquisition GenBank: BC001263
• Symbole du gène: SGK1
• Identification du gène (NCBI): 6446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.

Publications

Species	Application	Title
human		Environ Health Perspect; Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish; Authors - Rongyi Ye ; KD Validated
human		FASEB J; Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure.; Authors - Huaxin Zhou
mouse		J Innate Immun; Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.; Authors - Yunzhi Liu
mouse		J Immunol; Serum- and Glucocorticoid-Inducible Kinase 1 Promotes Alternative Macrophage Polarization and Restrains Inflammation through FoxO1 and STAT3 Signaling.; Authors - Junling Ren
mouse	IHC	J Transl Med; Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3; Authors - Xiangdong Li
human		Appl Biochem Biotechnol; Long Non-coding RNA ZFAS1 Regulates Fibrosis and Scortosis in the Cell Model of Diabetic Nephropathy Through miR-525-5p/SGK1 Axis; Authors - Langen Zhuang

{{ptg:RelatedPrimaryAntibodies}}