Anticorps Polyclonal de lapin anti-SGK1

• Phare
• Validé par KD/KO

SGK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 28454-1-AP

Synonymes

SGK, Serum/glucocorticoid-regulated kinase 1, Serine/threonine-protein kinase Sgk1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 28454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28454-1-AP (SGK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28454-1-AP (SGK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SGK1 antibody (28454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 28454-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SGK1 antibody (28454-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below

Informations sur le produit

28454-1-AP cible SGK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGK1 Protéine recombinante Ag29420
• Nom complet: serum/glucocorticoid regulated kinase 1
• Masse moléculaire calculée: 431 aa, 49 kDa
• Poids moléculaire observé: 42-50 kDa
• Numéro d’acquisition GenBank: BC001263
• Symbole du gène: SGK1
• Identification du gène (NCBI): 6446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Serum- and glucocorticoid-induced kinase 1 (SGK1),also named as SGK, is a S/T protein kinase that belongs to the AGC (cAMP-dependent, cGMP-dependent, and protein kinase C) kinase family. It is expressed in many cell types and participates in numerous cellular processes and it is ubiquitinated and degraded at the ER membrane(PMID: 16847254). The N-terminal motif of SGK1 is critical for its ubiquitination and degradation(PMID:16817852 ). SGK and Akt are likely to phosphorylate related substrates, as they share a similar consensus phosphorylation site (RXRXXS/T)(PMID:11154281). It has 5 isoforms produced by alternative promoter usage and alternative splicing.

Publications

Species	Application	Title
human,mouse	WB	J Biol Chem; SGK1 negatively regulates inflammatory immune responses and protects against alveolar bone loss through modulation of TRAF3 activity.; Authors - Xiao Han ; KD Validated
human	WB	Int J Mol Sci; PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2; Authors - Qiongfeng Chen
human	WB	Front Oncol; Modeling of senescence-related chemoresistance in ovarian cancer using data analysis and patient-derived organoids; Authors - Xintong Cai
mouse	WB	Int J Biol Sci; Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12; Authors - Xianzheng Zhang
human	WB	Cell Death Dis; CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2; Authors - Di Wang
human	WB	Iran J Basic Med Sci; Quercetin is a foe in the heart by targeting the hERG potassium channel; Authors - Zihao Lu

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