Anticorps Polyclonal de lapin anti-SGK3

• Phare
• Validé par KD/KO

SGK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12699-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 12699-1-AP (same clone as 12699-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 12699-1-AP (same clone as 12699-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 12699-1-AP (same clone as 12699-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 12699-1-AP (same clone as 12699-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12699-1-AP (same clone as 12699-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12699-1-AP (same clone as 12699-1-PBS)

  IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 12699-1-AP (same clone as 12699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 12699-1-AP (same clone as 12699-1-PBS)

  Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12699-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12699-1-PBS cible SGK3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGK3 Protéine recombinante Ag3149
• Nom complet: serum/glucocorticoid regulated kinase family, member 3
• Masse moléculaire calculée: 496 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC015326
• Symbole du gène: SGK3
• Identification du gène (NCBI): 23678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.

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