Anticorps Polyclonal de lapin anti-SGLT1

SGLT1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30861-1-PBS

Synonymes

SLC5A1, High affinity sodium-glucose cotransporter

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Galerie de données de validation

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  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

  IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30861-1-PBS cible SGLT1 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGLT1 Protéine recombinante Ag29338
• Nom complet: solute carrier family 5 (sodium/glucose cotransporter), member 1
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: NM_000343
• Symbole du gène: SGLT1
• Identification du gène (NCBI): 6523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).

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