Anticorps Polyclonal de lapin anti-SGLT2

• Phare
• Validé par KD/KO

SGLT2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (1)

N° de cat : 24654-1-AP

Synonymes

Na(+)/glucose cotransporter 2, SGLT2, SLC5A2, Sodium/glucose cotransporter 2

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Galerie de données de validation

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  WB analysis using 24654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24654-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24654-1-AP

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 24654-1-AP (SGLT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24654-1-AP (SGLT2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 24654-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24654-1-AP (SGLT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A549, tissu rénal de rat
• Résultats positifs en IHC: tissu rénal humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 13 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

24654-1-AP cible SGLT2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGLT2 Protéine recombinante Ag19163
• Nom complet: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Masse moléculaire calculée: 672 aa, 73 kDa
• Poids moléculaire observé: 69-70 kDa
• Numéro d’acquisition GenBank: BC131542
• Symbole du gène: SLC5A2
• Identification du gène (NCBI): 6524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

Publications

Species	Application	Title
mouse	WB	Circulation; Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2; Authors - Sha Chen
human	IHC	Cancer Lett; SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway via the hnRNPK-YAP1 axis.; Authors - Dianyun Ren
human	WB	Mol Metab; Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment.; Authors - Nunzia D'Onofrio ; KD Validated
mouse	IHC	Int J Mol Sci; Redefining the Role of ADAM17 in Renal Proximal Tubular Cells and Its Implications in an Obese Mouse Model of Pre-Diabetes.; Authors - Vanesa Palau
mouse	IF	Metabolism; Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.; Authors - Hitomi Otomo
human	WB	Front Pharmacol; Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro.; Authors - Ting Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elina (Verified Customer) (03-04-2019)

The antibody is quite specific, used at a 1:200 dilution in mouse kidney. Comparison to a secondary only staining shows some background. Overall it appears to work well in paraffin embedded tissues, (5um thick).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse kidney

SGLT2 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:24654-1-AP)