Anticorps Polyclonal de lapin anti-SGLT2

SGLT2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28683-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28683-1-AP (same clone as 28683-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28683-1-AP (SGLT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28683-1-AP (SGLT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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  IF Staining of rat kidney using 28683-1-AP (same clone as 28683-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using SGLT2 antibody (28683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28683-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28683-1-PBS cible SGLT2 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGLT2 Protéine recombinante Ag30120
• Nom complet: solute carrier family 5 (sodium/glucose cotransporter), member 2
• Masse moléculaire calculée: 672 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC131542
• Symbole du gène: SGLT2
• Identification du gène (NCBI): 6524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

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