Anticorps Polyclonal de lapin anti-SGSM3

• Phare
• Validé par KD/KO

SGSM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20825-1-PBS

Synonymes

MAP, Merlin associated protein, Merlin-associated protein, RabGAPLP, Rab-GTPase-activating protein-like protein

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Galerie de données de validation

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  WB analysis using 20825-1-AP (same clone as 20825-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 20825-1-AP (same clone as 20825-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 20825-1-AP (same clone as 20825-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

  IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:2000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 20825-1-AP (same clone as 20825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 20825-1-AP (SGSM3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 20825-1-AP (same clone as 20825-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGSM3 antibody (20825-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SGSM3 antibody (20825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)

  Immunofluorescent analysis of Hela cells, using SGSM3 antibody 20825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20825-1-PBS cible SGSM3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGSM3 Protéine recombinante Ag14800
• Nom complet: small G protein signaling modulator 3
• Masse moléculaire calculée: 749 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC008078
• Symbole du gène: SGSM3
• Identification du gène (NCBI): 27352
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805).

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