Anticorps Polyclonal de lapin anti-SH2D2A

SH2D2A Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18539-1-PBS

Synonymes

F2771, SCAP, SH2 domain protein 2A, SH2 domain-containing adapter protein, SH2 domain-containing protein 2A

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Galerie de données de validation

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  WB analysis of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18539-1-AP (same clone as 18539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18539-1-AP (SH2D2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18539-1-AP (same clone as 18539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18539-1-AP (SH2D2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 18539-1-AP (same clone as 18539-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug SH2D2A Polyclonal antibody (18539-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18539-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18539-1-PBS cible SH2D2A dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH2D2A Protéine recombinante Ag13355
• Nom complet: SH2 domain protein 2A
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC012107
• Symbole du gène: SH2D2A
• Identification du gène (NCBI): 9047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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