Anticorps Polyclonal de lapin anti-SH2D3A

SH2D3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15641-1-PBS

Synonymes

UNQ175/PRO201, SH2 domain-containing protein 3A, NSP1, Novel SH2-containing protein 1

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Galerie de données de validation

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  WB analysis of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 15641-1-AP (same clone as 15641-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15641-1-AP (same clone as 15641-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15641-1-AP (SH2D3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 15641-1-AP (same clone as 15641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15641-1-AP (SH2D3A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15641-1-AP (same clone as 15641-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH2D3A antibody (15641-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15641-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15641-1-PBS cible SH2D3A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH2D3A Protéine recombinante Ag8132
• Nom complet: SH2 domain containing 3A
• Masse moléculaire calculée: 576 aa, 63 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC006281
• Symbole du gène: SH2D3A
• Identification du gène (NCBI): 10045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SH2 domain containing 3A (SH2D3A) Is is a novel SH2-containing protein 1 (NSP1) with proline-rich domains and therefore probably involved in the signal-transduction of some ligand-activated tyrosine kinase receptors. Three phosphorylation sites of NSP1 have been found to date. NSP1 may be located at cell membrane play a role in JNK activation.

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