Anticorps Polyclonal de lapin anti-SH2D5

SH2D5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 55320-1-AP

Synonymes

SH2 domain-containing protein 5, SH2 domain containing 5

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 55320-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 55320-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 55320-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 55320-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 55320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 55320-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris
• Résultats positifs en IHC: tissu de cervelet humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55320-1-AP cible SH2D5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SH2 domain containing 5
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 43-47 kDa
• Numéro d’acquisition GenBank: NM_001103160
• Symbole du gène: SH2D5
• Identification du gène (NCBI): 400745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.

{{ptg:RelatedPrimaryAntibodies}}