Anticorps Polyclonal de lapin anti-SH2D5

SH2D5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55320-1-PBS

Synonymes

SH2 domain-containing protein 5, SH2 domain containing 5

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Galerie de données de validation

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  WB analysis of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 55320-1-AP (same clone as 55320-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 55320-1-AP (same clone as 55320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 55320-1-AP (same clone as 55320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55320-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55320-1-PBS cible SH2D5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SH2 domain containing 5
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 43-47 kDa
• Numéro d’acquisition GenBank: NM_001103160
• Symbole du gène: SH2D5
• Identification du gène (NCBI): 400745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.

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