Anticorps Polyclonal de lapin anti-SH3BP5

• Phare
• Validé par KD/KO

SH3BP5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11127-2-AP

Synonymes

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

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Galerie de données de validation

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  WB analysis of Raji using 11127-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Applications testées

• Résultats positifs en WB: cellules Raji
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:500
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below

Informations sur le produit

11127-2-AP cible SH3BP5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH3BP5 Protéine recombinante Ag1592
• Nom complet: SH3-domain binding protein 5 (BTK-associated)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 32 kDa, 50-83 kDa
• Numéro d’acquisition GenBank: BC010123
• Symbole du gène: SH3BP5
• Identification du gène (NCBI): 9467
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.

Publications

Species	Application	Title
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	J Hepatol; Sab (Sh3bp5) dependence of JNK mediated inhibition of mitochondrial respiration in palmitic acid induced hepatocyte lipotoxicity.; Authors - Sanda Win ; KD Validated
mouse	WB	Cell Death Dis; JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.; Authors - S Win ; KD Validated
human,mouse	WB	Front Pharmacol; Scoparone Improves Nonalcoholic Steatohepatitis Through Alleviating JNK/Sab Signaling Pathway-Mediated Mitochondrial Dysfunction.; Authors - Yuwei Jiang ; KD Validated
human	WB	Biomolecules; Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.; Authors - Minjing Li ; KD Validated
mouse	WB	Biomed Pharmacother; Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway; Authors - Lei Gu