Anticorps Polyclonal de lapin anti-SH3D19

SH3D19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 21499-1-AP

Synonymes

ADAM binding protein Eve 1, ADAM-binding protein Eve-1, EBP, EEN binding protein, EEN-binding protein

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Galerie de données de validation

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  WB analysis of HT-1080 using 21499-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 21499-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 21499-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21499-1-AP

  Immunofluorescent analysis of HepG2 cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 21499-1-AP

  Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules Y79
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21499-1-AP cible SH3D19 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH3D19 Protéine recombinante Ag15915
• Nom complet: SH3 domain containing 19
• Masse moléculaire calculée: 790 aa, 87 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC108890
• Symbole du gène: SH3D19
• Identification du gène (NCBI): 152503
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3D19, also known as EBP, Eve-1, encodes a multiple SH3 domain-containing protein. SH3D19 plays a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. SH3D19 is involved in the suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1(PMID: 14551139). SH3D19 is abundantly expressed in skeletal muscle and heart(PMID: 15280379).

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