Anticorps Monoclonal anti-SH3GLB2

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4B1A10

N° de cat : 68325-1-PBS

Synonymes

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Galerie de données de validation

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  WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

  IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68325-1-PBS cible SH3GLB2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SH3GLB2 Protéine recombinante Ag8932
• Nom complet: SH3-domain GRB2-like endophilin B2
• Masse moléculaire calculée: 395 aa, 44 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC014635
• Symbole du gène: SH3GLB2
• Identification du gène (NCBI): 56904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

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