Anticorps Polyclonal de lapin anti-SH3TC2

SH3TC2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 27905-1-AP

Synonymes

CMT4C, PP12494, SH3 domain and tetratricopeptide repeat-containing protein 2

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Galerie de données de validation

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  IHC staining of human placenta using 27905-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27905-1-AP (SH3TC2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27905-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27905-1-AP (SH3TC2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27905-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27905-1-AP (SH3TC2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SH3TC2 antibody (27905-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of U2OS using 27905-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SH3TC2 antibody (27905-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27905-1-AP cible SH3TC2 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH3TC2 Protéine recombinante Ag27352
• Nom complet: SH3 domain and tetratricopeptide repeats 2
• Masse moléculaire calculée: 1288 aa, 145 kDa
• Numéro d’acquisition GenBank: BC113879
• Symbole du gène: SH3TC2
• Identification du gène (NCBI): 79628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SH3TC2 protein (also known as KIAA1985 protein) is an adaptor protein involved in myelin formation in the peripheral nervous system. This protein contains multiple functional domains, including the SH3 (Src homology 3) and TPR (tetratricopeptide repeat) domains, which mediate protein-protein interactions. It is highly expressed in Schwann cells and is crucial for maintaining the stability of myelin structure. Clinically, mutations in the SH3TC2 gene are associated with Charcot-Marie-Tooth disease type 4C (CMT4C), an autosomal recessive peripheral neuropathy characterized by progressive demyelination of motor and sensory nerves, limb weakness, and loss of sensation. Studies have shown that the absence or dysfunction of SH3TC2 protein can lead to disordered signaling pathways in Schwann cells (such as Rab11-mediated membrane transport defects), affecting myelin development and repair.

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