Anticorps Polyclonal de lapin anti-SHC1

• Phare
• Validé par KD/KO

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12496-1-PBS

Synonymes

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

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Galerie de données de validation

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  WB analysis using 12496-1-AP (same clone as 12496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12496-1-AP (same clone as 12496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12496-1-AP (same clone as 12496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12496-1-PBS cible SHC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHC1 Protéine recombinante Ag3165
• Nom complet: SHC (Src homology 2 domain containing) transforming protein 1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa, 52 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC014158
• Symbole du gène: SHC1
• Identification du gène (NCBI): 6464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

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