Anticorps Polyclonal de lapin anti-SHD

SHD Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 20814-1-AP

Synonymes

SHD

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Galerie de données de validation

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  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 20814-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20814-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 20814-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Neuro-2a using 20814-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Neuro-2a, cellules NIH/3T3, cellules SMMC-7721
• Résultats positifs en IF/ICC: cellules Neuro-2a

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20814-1-AP cible SHD dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHD Protéine recombinante Ag14794
• Nom complet: Src homology 2 domain containing transforming protein D
• Masse moléculaire calculée: 340 aa, 38 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC007206
• Symbole du gène: SHD
• Identification du gène (NCBI): 56961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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