Anticorps Recombinant de lapin anti-Shh

Shh Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240057E7

N° de cat : 83280-1-PBS

Synonymes

M100081, Hxl3, Hx, Hhg1, Dsh

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Galerie de données de validation

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  WB analysis using 83280-1-RR (same clone as 83280-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 83280-1-RR (same clone as 83280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83280-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83280-1-PBS cible Shh dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sonic hedgehog
• Masse moléculaire calculée: 48KD
• Poids moléculaire observé: 19-50 kDa
• Numéro d’acquisition GenBank: NM_009170.3
• Symbole du gène: Shh
• Identification du gène (NCBI): 20423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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