Anticorps Recombinant de lapin anti-SHH

SHH Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250549A9

N° de cat : 86106-1-PBS

Synonymes

EC:3.1.-.-, HHG 1, HHG1, HHG-1, HLP3

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Galerie de données de validation

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  WB analysis of mouse liver using 86106-1-RR (same clone as 86106-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 86106-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 86106-1-RR (same clone as 86106-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 86106-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86106-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86106-1-PBS cible SHH dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SHH Protéine recombinante Eg4010
• Nom complet: sonic hedgehog homolog (Drosophila)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: NM_000193
• Symbole du gène: SHH
• Identification du gène (NCBI): 6469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SHH, also named as HHG-1, belongs to the hedgehog family. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. It regulates another target, the gli oncogene. The Shh protein is synthesized as a 45-kDa precursor that undergoes an autocatalytic processing event that produces a 19-kDa N-terminal product, responsible for all signaling activities, and a 25-kDa C-terminal fragment (PMID:10753901, PMID: 16282375). SHH can be detected precursor protein as 48-51-kDa polypeptides (PMID: 15292211).

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