Anticorps Polyclonal de lapin anti-SHMT2

• Phare
• Validé par KD/KO

SHMT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(28)

N° de cat : 11099-1-AP

Synonymes

EC:2.1.2.1, SHMT

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Galerie de données de validation

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  WB analysis using 11099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11099-1-AP

  WB result of SHMT2 antibody (11099-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHMT2 transfected HepG2 cells.

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  WB analysis of mouse kidney using 11099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11099-1-AP (SHMT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse brain using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11099-1-AP (SHMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11099-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11099-1-AP (SHMT2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 11099-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SHMT2 antibody (11099-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

11099-1-AP cible SHMT2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHMT2 Protéine recombinante Ag1584
• Nom complet: serine hydroxymethyltransferase 2 (mitochondrial)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC011911
• Symbole du gène: SHMT2
• Identification du gène (NCBI): 6472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	IHC	EMBO Mol Med; Proteomics and personalized PDX models identify treatment for a progressive malignancy within an actionable timeframe; Authors - Georgina D Barnabas
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB,IHC	Am J Respir Cell Mol Biol; Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis.; Authors - Robert B Hamanaka
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated

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