Anticorps Monoclonal anti-SHMT2

SHMT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A2H9

N° de cat : 67980-1-PBS

Synonymes

3A2H9, EC:2.1.2.1, SHMT

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Galerie de données de validation

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  WB analysis using 67980-1-Ig (same clone as 67980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67980-1-Ig (same clone as 67980-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT2 antibody (67980-1-Ig, Clone: 3A2H9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67980-1-PBS cible SHMT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SHMT2 Protéine recombinante Ag1584
• Nom complet: serine hydroxymethyltransferase 2 (mitochondrial)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC011911
• Symbole du gène: SHMT2
• Identification du gène (NCBI): 6472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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