Anticorps Monoclonal anti-SIAE

SIAE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G5F7

N° de cat : 66522-1-PBS

Synonymes

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

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Galerie de données de validation

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  WB analysis using 66522-1-Ig (same clone as 66522-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 66522-1-Ig (same clone as 66522-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66522-1-Ig (same clone as 66522-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 66522-1-Ig (same clone as 66522-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66522-1-PBS cible SIAE dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIAE Protéine recombinante Ag14624
• Nom complet: sialic acid acetylesterase
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC068450
• Symbole du gène: SIAE
• Identification du gène (NCBI): 54414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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