Anticorps Polyclonal de lapin anti-SIAH1

• Phare
• Validé par KD/KO

SIAH1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13886-1-AP

Synonymes

hSIAH1, HUMSIAH, Seven in absentia homolog 1, Siah 1, Siah 1a, SIAH1

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Galerie de données de validation

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  WB analysis of HeLa using 13886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13886-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13886-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13886-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 13886-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13886-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13886-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 13886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13886-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules COLO 320, cellules HepG2, cellules Jurkat, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

13886-1-AP cible SIAH1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIAH1 Protéine recombinante Ag4336
• Nom complet: seven in absentia homolog 1 (Drosophila)
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC035562
• Symbole du gène: SIAH1
• Identification du gène (NCBI): 6477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

Publications

Species	Application	Title
human	WB	Cell Rep; Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis.; Authors - Yi Zeng
human	WB	Exp Cell Res; Siah1 promotes the proliferation of NSCLC cells through ubiquitinating and stabilizing Notch1; Authors - Yan Liu ; KD Validated
human	WB	iScience; Cathepsin K promotes the proliferation of hepatocellular carcinoma cells through induction of SIAH1 ubiquitination and degradation; Authors - Chengming Zhang
human	WB	J Exp Clin Cancer Res; GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway; Authors - Haijun Bao