Anticorps Polyclonal de lapin anti-SIAH1

• Phare
• Validé par KD/KO

SIAH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13886-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13886-1-AP (same clone as 13886-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 13886-1-AP (same clone as 13886-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW264.7 using 13886-1-AP (same clone as 13886-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 13886-1-AP (same clone as 13886-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 13886-1-AP (same clone as 13886-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13886-1-AP (same clone as 13886-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13886-1-PBS cible SIAH1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIAH1 Protéine recombinante Ag4336
• Nom complet: seven in absentia homolog 1 (Drosophila)
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC035562
• Symbole du gène: SIAH1
• Identification du gène (NCBI): 6477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

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