Anticorps Polyclonal de lapin anti-SIAH2

• Phare
• Validé par KD/KO

SIAH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 12651-1-AP

Synonymes

5N16, E3 ubiquitin-protein ligase SIAH2, EC:2.3.2.27, hSiah2, RING-type E3 ubiquitin transferase SIAH2

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Galerie de données de validation

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  WB analysis of mouse brain using 12651-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12651-1-AP (SIAH2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis using 12651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12651-1-AP (SIAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12651-1-AP

  IP result of anti-SIAH2 (IP:12651-1-AP, 4ug; Detection:12651-1-AP 1:500) with MCF-7 cells lysate 760 ug.

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  IHC staining of mouse brain using 12651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12651-1-AP (SIAH2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12651-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12651-1-AP (SIAH2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 12651-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SIAH2 antibody (12651-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules MDA-MB-231, tissu cérébral de souris
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Informations sur le produit

12651-1-AP cible SIAH2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIAH2 Protéine recombinante Ag3342
• Nom complet: seven in absentia homolog 2 (Drosophila)
• Masse moléculaire calculée: 324 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC013082
• Symbole du gène: SIAH2
• Identification du gène (NCBI): 6478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian Seven-in-absentia homolog 2 (SIAH2), is a RING finger type ubiquitin ligase with a catalytic RING domain on its N-terminus, followed by two zinc fingers and a C-terminal substrate binding domain. Siah is a mammalian homolog of Sina, a Drosophila protein functioning in eye development. Mammals express two isoforms, Siah1 and 2, and mice exhibit two Siah1 isotypes, 1a and 1b. SIAH2 indirectly regulates the level of HIF-1α by promoting degradation of PHDs by UPS. SIAH2, in turn, is positively regulated by hypoxia-dependent transcriptional and posttranscriptional mechanisms.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo ; KD Validated
human	WB	Nucleic Acids Res; P-TEFb is degraded by Siah1/2 in quiescent cells.; Authors - Fang Huang ; KD Validated
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
human	WB,IP	Int J Biol Sci; Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells; Authors - Xiaofeng Dai
		Cell Death Dis; PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.; Authors - Yingying Zhang
mouse	WB,IF	Int Immunopharmacol; Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments; Authors - Guoyao Ou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patrick (Verified Customer) (11-13-2019)

Couldn't detect endogenous or overexpressed SIAH2

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 293T

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