Anticorps Polyclonal de lapin anti-SIDT1

SIDT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55352-1-PBS

Synonymes

SID1 transmembrane family member 1, SID1, SID 1

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Galerie de données de validation

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  WB analysis using 55352-1-AP (same clone as 55352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55352-1-AP (SIDT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55352-1-AP (same clone as 55352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 55352-1-AP (same clone as 55352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 55352-1-AP (same clone as 55352-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SIDT1 antibody (55352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55352-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55352-1-PBS cible SIDT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SID1 transmembrane family, member 1
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 95-110 kDa
• Numéro d’acquisition GenBank: NM_017699
• Symbole du gène: SIDT1
• Identification du gène (NCBI): 54847
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIDT1, also known as a human orthologue of SID-1, belongs to the SID family which is a highly conserved group of transmembrane channel-like proteins. As a transmembrane channel for intercellular communication, SIDT1 was found to facilitate small interfering RNA (siRNA) or miRNA uptake and also involved in cholesterol transport in human systems. SIDT1 mRNA and protein were mainly detected in the stomach and large intestine (including the cecum, colon and rectum) but not in the small intestine (especially the duodenum). Notably, SIDT1 was mainly located in gastric surface mucous cells (pit cells) but barely expressed in the parietal or chief cells. The SIDT1 gene encodes an 827 amino acid protein with a calculated molecular mass of 94 kDa. (PMID: 31632087, PMID: 32801357, PMID: 22174421)

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