Anticorps Polyclonal de lapin anti-SIGIRR

SIGIRR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 27828-1-AP

Synonymes

Toll/interleukin-1 receptor 8, Toll/interleukin 1 receptor 8, TIR8, Single immunoglobulin domain-containing IL1R-related protein, Single Ig IL-1R-related molecule

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Galerie de données de validation

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  WB analysis of mouse lung using 27828-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27828-1-AP (SIGIRR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 27828-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27828-1-AP (SIGIRR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris,
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

27828-1-AP cible SIGIRR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIGIRR Protéine recombinante Ag25850
• Nom complet: single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC025953
• Symbole du gène: SIGIRR
• Identification du gène (NCBI): 59307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIGIRR (also known as TIR8) is a member of the toll-like receptor (TLR)/interleukin 1 receptor (IL-1R) superfamily, with a single immunoglobulin extracellular domain and a TIR (Toll/IL-1R) intracellular domain. SIGIRR functions as a negative regulator for IL-1 and LPS signaling, through its interaction with the TLR4 and IL-1R complex. SIGIRR is an important modulator of intestinal epithelial homeostasis and a key regulator of mucosal immunity, maintaining microbial tolerance of the intestinal epithelial layer (PMID: 17398123). The molecular weight of the glycosylated SIGIRR was found to be in the range 50-80 kDa (PMID: 10346978; 17495864).

Publications

Species	Application	Title
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
human	WB	Cell Death Dis; IL-37b alleviates endothelial cell apoptosis and inflammation in Kawasaki disease through IL-1R8 pathway.; Authors - Chang Jia
human,mouse	WB,ELISA	Ital J Pediatr; SIGIRR-caspase-8 signaling mediates endothelial apoptosis in Kawasaki disease; Authors - Zhengwang Wen
human	WB,IF	J Control Release; AAV9-delivery of interleukin-37b gene prevents recurrent herpetic stromal keratitis via the SIGIRR pathway in mice; Authors - Jing Wu

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