Anticorps Polyclonal de lapin anti-Siglec-9

Siglec-9 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13377-1-AP

Synonymes

SIGLEC9, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9, Siglec 9

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Galerie de données de validation

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  WB analysis of HL-60 using 13377-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13377-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13377-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13377-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13377-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13377-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 13377-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Siglec-9 antibody (13377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules A375, cellules U-937, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13377-1-AP cible Siglec-9 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Siglec-9 Protéine recombinante Ag4201
• Nom complet: sialic acid binding Ig-like lectin 9
• Masse moléculaire calculée: 463 aa, 50 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC035365
• Symbole du gène: Siglec-9
• Identification du gène (NCBI): 27180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

Publications

Species	Application	Title
human	IF	Nat Cancer; Siglec-9 acts as an immune-checkpoint molecule on macrophages in glioblastoma, restricting T-cell priming and immunotherapy response; Authors - Yan Mei
human	WB,IHC,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: Prostate

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