Anticorps Polyclonal de lapin anti-Siglec-9

Siglec-9 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 13377-1-PBS

Synonymes

SIGLEC9, UNQ668/PRO1302, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9

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Galerie de données de validation

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  WB analysis of HL-60 using 13377-1-AP (same clone as 13377-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 13377-1-AP (same clone as 13377-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 13377-1-AP (same clone as 13377-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 13377-1-AP (same clone as 13377-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 13377-1-AP (same clone as 13377-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Siglec-9 antibody (13377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13377-1-PBS cible Siglec-9 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Siglec-9 Protéine recombinante Ag4201
• Nom complet: sialic acid binding Ig-like lectin 9
• Masse moléculaire calculée: 463 aa, 50 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC035365
• Symbole du gène: Siglec-9
• Identification du gène (NCBI): 27180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

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