Anticorps Recombinant de lapin anti-SIGMAR1

SIGMAR1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242621C6

N° de cat : 85064-1-PBS

Synonymes

242621C6, AAG8, Aging-associated gene 8 protein, hSigmaR1, OPRS1

---

Galerie de données de validation

• 

  WB analysis using 85064-1-RR (same clone as 85064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85064-1-RR (SIGMAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85064-1-RR (same clone as 85064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85064-1-RR (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85064-1-RR (same clone as 85064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85064-1-RR (SIGMAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01809-1

  Cytometric bead array standard curve of MP01809-1, SIGMAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85064-2-PBS. Detection antibody: 85064-1-PBS. Standard: Ag7351. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 85064-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85064-1-RR against Human SIGMAR1 were performed. The affinity constant is 1.54 nM.

---

Informations sur le produit

85064-1-PBS cible SIGMAR1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SIGMAR1 Protéine recombinante Ag7351
• Nom complet: sigma non-opioid intracellular receptor 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC004899
• Symbole du gène: SIGMAR1
• Identification du gène (NCBI): 10280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 25 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

{{ptg:RelatedPrimaryAntibodies}}