Anticorps Polyclonal de lapin anti-TCEA1

• Phare
• Validé par KD/KO

TCEA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17825-1-AP

Synonymes

GTF2S, SII, SII TFIIS, TCEA, TCEA1, TF2S, TFIIS

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Galerie de données de validation

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  WB analysis of Sp2/0 using 17825-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17825-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17825-1-AP (TCEA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17825-1-AP

  IP result of anti-TCEA1 (IP:17825-1-AP, 4ug; Detection:17825-1-AP 1:500) with HeLa cells lysate 520ug.

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  IHC staining of human brain using 17825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17825-1-AP (TCEA1 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human brain using 17825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17825-1-AP (TCEA1 antibody) at dilution of 1:400 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Sp2/0, cellules HeLa, cellules SGC-7901
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

17825-1-AP cible TCEA1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCEA1 Protéine recombinante Ag12199
• Nom complet: transcription elongation factor A (SII), 1
• Masse moléculaire calculée: 301 aa, 34 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC072460
• Symbole du gène: TCEA1
• Identification du gène (NCBI): 6917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

Publications

Species	Application	Title
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Am J Cancer Res; Abnormal expression of YEATS4 associates with poor prognosis and promotes cell proliferation of hepatic carcinoma cell by regulation the TCEA1/DDX3 axis.; Authors - Song You

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