• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Recombinant de lapin anti-SII-TFIIS,TCEA1

SII-TFIIS,TCEA1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243155H8

N° de cat : 85664-4-PBS

Synonymes

TCEA1, GTF2S, SII, SII TFIIS, TCEA

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    WB analysis using 85664-4-RR (same clone as 85664-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of HeLa using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of A431 using 85664-4-RR (same clone as 85664-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

Informations sur le produit

85664-4-PBS cible SII-TFIIS,TCEA1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène SII-TFIIS,TCEA1 Protéine recombinante Ag12199
Nom complet transcription elongation factor A (SII), 1
Masse moléculaire calculée 301 aa, 34 kDa
Poids moléculaire observé40 kDa
Numéro d’acquisition GenBankBC072460
Symbole du gène TCEA1
Identification du gène (NCBI) 6917
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

{{ptg:RelatedPrimaryAntibodies}}