Anticorps Polyclonal de lapin anti-Snf1lk

• Phare
• Validé par KD/KO

Snf1lk Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (2)

N° de cat : 51045-1-AP

Synonymes

Sik1, EC:2.7.11.1, HRT-20, Msk, Myocardial SNF1-like kinase

---

Galerie de données de validation

• 

  WB analysis of A549 using 51045-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 51045-1-AP

  WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

• 

  WB analysis using 51045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51045-1-AP (Snf1lk Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 51045-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Informations sur le produit

51045-1-AP cible Snf1lk dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Snf1lk Protéine recombinante Ag3913
• Nom complet: salt inducible kinase 1
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85-100 kDa
• Numéro d’acquisition GenBank: NM_010831
• Symbole du gène: Sik1
• Identification du gène (NCBI): 17691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

Publications

Species	Application	Title
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
human	WB,IHC	iScience; Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus; Authors - Jie Ning ; KD Validated
human	WB,IF	J Neuroinflammation; High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.; Authors - Mengyan Hu
human	WB	J Cell Physiol; ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes; Authors - Xueyi Chen
rat,mouse	IHC,IF	J Cereb Blood Flow Metab; Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.; Authors - Cheng Jian J
human	WB	J Leukoc Biol; SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.; Authors - Maria Stella Lombardi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

SD (Verified Customer) (07-05-2024)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

Snf1lk Antibody Western Blot validation (1000 dilution) in A549 (Cat no:51045-1-AP)

FH

P (Verified Customer) (05-16-2024)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 500
• Cell Tissue Type: AC16

Snf1lk Antibody Western Blot validation (500 dilution) in AC16 (Cat no:51045-1-AP)

{{ptg:RelatedPrimaryAntibodies}}