Anticorps Polyclonal de lapin anti-SIM2

SIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21069-1-PBS

Synonymes

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

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Galerie de données de validation

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  WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21069-1-PBS cible SIM2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIM2 Protéine recombinante Ag15204
• Nom complet: single-minded homolog 2 (Drosophila)
• Masse moléculaire calculée: 667 aa, 73 kDa
• Poids moléculaire observé: 60-64 kDa
• Numéro d’acquisition GenBank: BC110444
• Symbole du gène: SIM2
• Identification du gène (NCBI): 6493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

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